Background: microRNA-22 (miR-22) was frequently altered in numerous cancers and was involved in various cellular processes related to carcinogenesis. However, the role of miR-22 in cervical cancer haven’t been investigated.
Methods: 62 pairs of fresh cervical cancer tissue were collected between May 2012 and March 2015. Real-time quantitative RT-PCR assay was performed to evaluate the expression levels of miR-22. The survival curves were determined using the Kaplan-Meier method and Cox regression for statistics. The roles of miR-22 in cell proliferation, migration and invasion were identified using miR-22 mimic-transfected cells. In addition, the regulatory effect of miR-22 on suppressor of cancer cell invasion (SCAI) was evaluated using qRT-PCR a dual luciferase reporter assay.
Results: miR-22 expression in cervical cancer tissues was significantly lower than that in normal tissues (mean
Conclusions: Our data demonstrated that the expression of miR-22 was downregulated in cervical cancer, and associated with overall survival as well as progression-free survival, suggesting that miR-22 could serve as an efficient prognostic factor for cervical cancer patients